Bidirectional synaptic transmission in Necturus taste buds
نویسندگان
چکیده
منابع مشابه
Bidirectional synaptic transmission in Necturus taste buds.
Pairs of taste cells were impaled with intracellular recording microelectrodes in intact taste buds in slices of Necturus lingual epithelium. Applying short pulses of 140 mM KCl or 200 mM CaCl2 solutions to the apical pore elicited receptor potentials in taste receptor cells. Chemostimulation of receptor cells elicited postsynaptic responses in basal cells in the taste bud. Postsynaptic respons...
متن کاملIdentification of electrophysiologically distinct cell subpopulations in Necturus taste buds
We used the patch clamp technique to record from taste cells in thin transverse slices of lingual epithelium from Necturus maculosus. In this preparation, the epithelial polarity and the cellular organization of the taste buds, as well as the interrelationships among cells within the taste bud, were preserved. Whole-cell recording, combined with cell identification using Lucifer yellow, allowed...
متن کاملSynaptic connections in developing and adult rat taste buds.
Synaptic connections in rat taste buds closely resemble synapses found in the CNS. Taste bud synapses are characterized by parallel, apposed, thickened membranes that are separated by a cleft ranging from 16 to 30 nm. The putative synaptic vesicles are of two types: small, clear vesicles (40–70 nm) and large, dense-cored vesicles (90–120 nm). The small, clear vesicles predominate at most synaps...
متن کاملMembrane properties of two types of basal cells in Necturus taste buds.
Necturus taste buds contain two types of basal cells: presumptive stem cells and Merkel-like basal cells. Both types of basal cells are small round cells located at the base of the taste bud, indistinguishable from each other with light microscopy. However, with electron microscopy, autoradiography, or immunocytochemistry, these two types of basal cells can be easily distinguished. We isolated ...
متن کاملEstimation of the junctional resistance between electrically coupled receptor cells in Necturus taste buds
Junctional resistance between coupled receptor cells in Necturus taste buds was estimated by modeling the results from single patch pipette voltage clamp studies on lingual slices. The membrane capacitance and input resistance of coupled taste receptor cells were measured to monitor electrical coupling and the results compared with those calculated by a simple model of electrically coupled tast...
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ژورنال
عنوان ژورنال: The Journal of Neuroscience
سال: 1994
ISSN: 0270-6474,1529-2401
DOI: 10.1523/jneurosci.14-06-03791.1994